[Molecular mechanisms of reversions to the ts+ -phenotype of cold-adapted influenza virus A strains--attenuation donors for live influenza reassortant vaccines].
Identifieur interne : 002E03 ( Main/Exploration ); précédent : 002E02; suivant : 002E04[Molecular mechanisms of reversions to the ts+ -phenotype of cold-adapted influenza virus A strains--attenuation donors for live influenza reassortant vaccines].
Auteurs : S G Markushin ; I I Akopova ; I B Koptiaeva ; T M Tsfasman ; Iu Z. GendonSource :
- Voprosy virusologii [ 0507-4088 ]
Descripteurs français
- KwdFr :
- Adaptation physiologique, Animaux, Embryon de poulet, Passage en série, Protéines virales (génétique), Recombinaison génétique, Réaction de polymérisation en chaîne, Réplication virale, Sous-type H2N2 du virus de la grippe A (génétique), Sous-type H2N2 du virus de la grippe A (physiologie), Suppression génétique, Température élevée, Test de complémentation, Virus recombinants (génétique), Virus recombinants (physiologie).
- MESH :
- génétique : Protéines virales, Sous-type H2N2 du virus de la grippe A, Virus recombinants.
- physiologie : Sous-type H2N2 du virus de la grippe A, Virus recombinants.
- Adaptation physiologique, Animaux, Embryon de poulet, Passage en série, Recombinaison génétique, Réaction de polymérisation en chaîne, Réplication virale, Suppression génétique, Température élevée, Test de complémentation.
English descriptors
- KwdEn :
- Adaptation, Physiological, Animals, Chick Embryo, Genetic Complementation Test, Hot Temperature, Influenza A Virus, H2N2 Subtype (genetics), Influenza A Virus, H2N2 Subtype (physiology), Polymerase Chain Reaction, Reassortant Viruses (genetics), Reassortant Viruses (physiology), Recombination, Genetic, Serial Passage, Suppression, Genetic, Viral Proteins (genetics), Virus Replication.
- MESH :
- chemical , genetics : Viral Proteins.
- genetics : Influenza A Virus, H2N2 Subtype, Reassortant Viruses.
- physiology : Influenza A Virus, H2N2 Subtype, Reassortant Viruses.
- Adaptation, Physiological, Animals, Chick Embryo, Genetic Complementation Test, Hot Temperature, Polymerase Chain Reaction, Recombination, Genetic, Serial Passage, Suppression, Genetic, Virus Replication.
Abstract
A ts+ revertant of cold-adapted (ca) strain A/Leningrad/134/47/57--the attenuation donor for live influenza reassortant vaccines--was obtained by passages of the ca strain in chick embryos at nonpermissive temperatures. The ts+ revertant acquired the ability to grow in chick embryos at 40 degrees C and lost the capacity to reproduce there at 25 degrees C. A complementation-recombination test using the fowl plague virus (FPV0 ts-mutants showed the loss of the ts-phenotype in the RNA-segments of ts+ revertants' genome coding for PB2, NP, and NS (NS2) proteins. However, PCR-restriction analysis revealed a true reversion in RNA-segment coding for PB2 protein only. All the investigated mutations in the ts+ revertant genome were preserved. This phenomenon could be explained by the appearance of intragenic and extragenic suppression mutations in the ts+ revertant genome. The data of the complementation-recombination test suggest that reversion of ts-phenotype occurs more frequently due to extra- or intragenic suppression rather than as a result of a true mutation loss. Estimation of the genetic stability of vaccine ca strains of influenza virus should be based on the combined use of PCR-restriction and complementation tests.
PubMed: 17087060
Affiliations:
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Le document en format XML
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Gendon</name></author></analytic><series><title level="j">Voprosy virusologii</title><idno type="ISSN">0507-4088</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adaptation, Physiological</term><term>Animals</term><term>Chick Embryo</term><term>Genetic Complementation Test</term><term>Hot Temperature</term><term>Influenza A Virus, H2N2 Subtype (genetics)</term><term>Influenza A Virus, H2N2 Subtype (physiology)</term><term>Polymerase Chain Reaction</term><term>Reassortant Viruses (genetics)</term><term>Reassortant Viruses (physiology)</term><term>Recombination, Genetic</term><term>Serial Passage</term><term>Suppression, Genetic</term><term>Viral Proteins (genetics)</term><term>Virus Replication</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adaptation physiologique</term><term>Animaux</term><term>Embryon de poulet</term><term>Passage en série</term><term>Protéines virales (génétique)</term><term>Recombinaison génétique</term><term>Réaction de polymérisation en chaîne</term><term>Réplication virale</term><term>Sous-type H2N2 du virus de la grippe A (génétique)</term><term>Sous-type H2N2 du virus de la grippe A (physiologie)</term><term>Suppression génétique</term><term>Température élevée</term><term>Test de complémentation</term><term>Virus recombinants (génétique)</term><term>Virus recombinants (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A Virus, H2N2 Subtype</term><term>Reassortant Viruses</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines virales</term><term>Sous-type H2N2 du virus de la grippe A</term><term>Virus recombinants</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Sous-type H2N2 du virus de la grippe A</term><term>Virus recombinants</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Influenza A Virus, H2N2 Subtype</term><term>Reassortant Viruses</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adaptation, Physiological</term><term>Animals</term><term>Chick Embryo</term><term>Genetic Complementation Test</term><term>Hot Temperature</term><term>Polymerase Chain Reaction</term><term>Recombination, Genetic</term><term>Serial Passage</term><term>Suppression, Genetic</term><term>Virus Replication</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adaptation physiologique</term><term>Animaux</term><term>Embryon de poulet</term><term>Passage en série</term><term>Recombinaison génétique</term><term>Réaction de polymérisation en chaîne</term><term>Réplication virale</term><term>Suppression génétique</term><term>Température élevée</term><term>Test de complémentation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A ts+ revertant of cold-adapted (ca) strain A/Leningrad/134/47/57--the attenuation donor for live influenza reassortant vaccines--was obtained by passages of the ca strain in chick embryos at nonpermissive temperatures. The ts+ revertant acquired the ability to grow in chick embryos at 40 degrees C and lost the capacity to reproduce there at 25 degrees C. A complementation-recombination test using the fowl plague virus (FPV0 ts-mutants showed the loss of the ts-phenotype in the RNA-segments of ts+ revertants' genome coding for PB2, NP, and NS (NS2) proteins. However, PCR-restriction analysis revealed a true reversion in RNA-segment coding for PB2 protein only. All the investigated mutations in the ts+ revertant genome were preserved. This phenomenon could be explained by the appearance of intragenic and extragenic suppression mutations in the ts+ revertant genome. The data of the complementation-recombination test suggest that reversion of ts-phenotype occurs more frequently due to extra- or intragenic suppression rather than as a result of a true mutation loss. Estimation of the genetic stability of vaccine ca strains of influenza virus should be based on the combined use of PCR-restriction and complementation tests.</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Akopova, I I" sort="Akopova, I I" uniqKey="Akopova I" first="I I" last="Akopova">I I Akopova</name><name sortKey="Gendon, Iu Z" sort="Gendon, Iu Z" uniqKey="Gendon I" first="Iu Z" last="Gendon">Iu Z. Gendon</name><name sortKey="Koptiaeva, I B" sort="Koptiaeva, I B" uniqKey="Koptiaeva I" first="I B" last="Koptiaeva">I B Koptiaeva</name><name sortKey="Markushin, S G" sort="Markushin, S G" uniqKey="Markushin S" first="S G" last="Markushin">S G Markushin</name><name sortKey="Tsfasman, T M" sort="Tsfasman, T M" uniqKey="Tsfasman T" first="T M" last="Tsfasman">T M Tsfasman</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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